Chlorhexidine

Chlorhexidine	Last updated: 30/08/2023
(Also known as: chlorohexidine)

Data alerts

The following alerts are based on the data in the tables below. An absence of an alert does not imply the substance has no implications for human health, biodiversity or the environment but just that we do not have the data to form a judgement.

Environmental fate	Ecotoxicity	Human health
Environmental fate Low alert: Drainflow: Non-mobile Warning: Significant data are missing	Ecotoxicity High alert: Daphnia acute ecotoxicity: High Warning: Significant data are missing	Human health Moderate alert: Mammals acute toxicity: Moderate; Reproduction/development effects Warning: Significant data are missing

GENERAL INFORMATION

Description

Used mainly as a used topical disinfectant cleanser for skin wounds and as an antibactericide for ear and gum infections killing both gram-positive and gram-negative microbes.

Availability status

Introduction & key dates

1953, UK

Examples of species treated

Cattle, Cats, Dogs

Chemical structure

Isomerism

Isomeric

Chemical formula

C₂₂H₃₀Cl₂N₁₀

Canonical SMILES

C1=CC(=CC=C1NC(=NC(=NCCCCCCN=C(N)N=C(N)NC2=CC=C(C=C2)Cl)N)N)Cl

Isomeric SMILES

C1=CC(=CC=C1N/C(=N/C(=NCCCCCCN=C(/N=C(/NC2=CC=C(C=C2)Cl)\N)N)N)/N)Cl

International Chemical Identifier key (InChIKey)

GHXZTYHSJHQHIJ-UHFFFAOYSA-N

International Chemical Identifier (InChI)

InChI=1S/C22H30Cl2N10/c23-15-5-9-17(10-6-15)31-21(27)33-19(25)29-13-3-1-2-4-14-30-20(26)34-22(28)32-18-11-7-16(24)8-12-18/h5-12H,1-4,13-14H2,(H5,25,27,29,31,33)(H5,26,28,30,32,34)

2D structure diagram/image available?

Yes

General status

Veterinary substance type

Antiseptic, Bactericide, Antimicrobial

Substance groups

Unclassified substance

Minimum active substance purity

Known relevant impurities

Substance origin

Synthetic

Mode of action

Disrupts the integrity of the cell membrane and causes the leakage of intracellular components of the organisms.

Molecular targets

[Bacterial cell wall]

CAS RN

55-56-1

EC number

200-238-7

CIPAC number

US EPA chemical code

PubChem CID

9552079

ATCvet Code

QD08AC52

Therapeutic Class

Dermatogiocals: Antiseptics and disinfectants

Controlled Drug?

Regulation 37/2010 MRL Classification

Allowable substance (Table 1: All food producing species)

Molecular mass

505.45

PIN (Preferred Identification Name)

IUPAC name

(1E)-2-[6-[[amino-[(E)-[amino-(4-chloroanilino)methylidene]amino]methylidene]amino]hexyl]-1-[amino-(4-chloroanilino)methylidene]guanidine

CAS name

1,6-bis(5-(p-chlorophenyl)biguanidino)hexane

Other status information

Relevant Environmental Water Quality Standards

Physical state

Colourless to pale yellow crystalline solid

Formulations

Property

Product

Manufacturer

Authorisation Route

UK Legal Class

Example products using this active

Action Actodine

Kilco Chemicals Ltd

UK National authorisation (IC)

Authorised veterinary medicine for general sale (AVM - GSL)

Deosan SummerTeatcare Plus ( 0.425%w/w ) Teat dip and Teat Spray Emulsion

JohnsonDiversey Ltd

UK National authorisation

Authorised veterinary medicine for general sale (AVM - GSL)

Propisderm 0.5 % w/w Teat dip solution

Hypred S.A.

UK National authorisation

Authorised veterinary medicine for general sale (AVM - GSL)

Sebolyse shampoo for dogs and cats

Dechra Veterinary Products A/S

UK National authorisation

Prescription only medicine to be authorised by a veterinarian (POM-V)

Formulation and application details

Available as solutions for topical application

ENVIRONMENTAL FATE

Property

Value

Source; quality score; and other information

Interpretation

Solubility - In water at 20 °C (mg l⁻¹)

800

High

Solubility - In organic solvents at 20 °C (mg l⁻¹)

Melting point (°C)

134

Boiling point (°C)

Degradation point (°C)

Flashpoint (°C)

Octanol-water partition coefficient at pH 7, 20 °C

1.20 X 10⁰⁰

Calculated

Log P

0.08

Low

Fat solubility of residues

Solubility

Data type

Density (g ml⁻¹)

1.07

Dissociation constant pKa) at 25 °C

10.78

at 25 °C

Vapour pressure at 20 °C (mPa)

2.64 X 10^-09

as acetate

Low volatility

Henry's law constant at 25 °C (Pa m³ mol⁻¹)

1.25e-25

as acetate

Non-volatile

Maximum UV-vis absorption L mol⁻¹ cm⁻¹

Surface tension (mN m⁻¹)

Refractive Index

Degradation

Property

Value

Source; quality score; and other information

Interpretation

Soil degradation (days) (aerobic)

DT₅₀ (typical)

DT₅₀ (lab at 20 °C)

DT₅₀ (field)

DT₉₀ (lab at 20 °C)

DT₉₀ (field)

Note

Manure DT₅₀ (days)

Aqueous photolysis DT₅₀ (days) at pH 7

Value

Note

Aqueous hydrolysis DT₅₀ (days) at 20 °C and pH 7

Value

Note

Water-sediment DT₅₀ (days)

Water phase only DT₅₀ (days)

Soil adsorption and mobility

Property

Value

Source; quality score; and other information

Interpretation

Linear

K_d

Non-mobile

K_oc

31600

Notes and range

Literature estimate

Freundlich

K_f

K_foc

¹/_n

Notes and range

pH sensitivity

Fate indices

Property

Value

Source; quality score; and other information

Interpretation

GUS leaching potential index

Bio-concentration factor

BCF (l kg⁻¹)

CT₅₀ (days)

Known metabolites

None

ECOTOXICOLOGY

Terrestrial ecotoxicology

Property

Value

Source; quality score; and other information

Interpretation

Mammals - Acute oral LD₅₀ (mg kg⁻¹)

> 1800

Mouse as the digluconate

Moderate

Mammals - Short term dietary NOEL

(mg kg⁻¹)

(ppm diet)

Mammals - Chronic 21d NOAEL (mg kg⁻¹ bw d⁻¹)

Birds - Acute LD₅₀ (mg kg⁻¹)

Birds - Short term dietary (LC₅₀/LD₅₀)

Birds - Chronic 21d NOEL (mg kg⁻¹ bw d⁻¹)

Earthworms - Acute 14 day LC₅₀ (mg kg⁻¹)

Earthworms - Chronic NOEC, reproduction (mg kg⁻¹)

Soil micro-organisms

Collembola

Acute LC₅₀ (mg kg⁻¹)

Chronic NOEC (mg kg⁻¹)

Non-target plants

Honeybees (Apis spp.)

Contact acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Oral acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Unknown mode acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Chronic

Bumblebees (Bombus spp.)

Contact acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Oral acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Mason bees (Osmia spp.)

Contact acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Oral acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Other bee species (1)

Acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg insect⁻¹)

Mode of exposure

Other bee species (2)

Acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg insect⁻¹)

Mode of exposure

Beneficial insects (Ladybirds)

Beneficial insects (Lacewings)

Beneficial insects (Parasitic wasps)

Beneficial insects (Predatory mites)

Beneficial insects (Ground beetles)

Aquatic ecotoxicology

Property

Value

Source; quality score; and other information

Interpretation

Fish - Acute 96 hour LC₅₀ (mg l⁻¹)

> 1.4

Danio rerio

Moderate

Fish - Chronic 21 day NOEC (mg l⁻¹)

Aquatic invertebrates - Acute 48 hour EC₅₀ (mg l⁻¹)

> 0.06

Daphnia magna as acetate

High

Aquatic invertebrates - Chronic 21 day NOEC (mg l⁻¹)

Aquatic crustaceans - Acute 96 hour LC₅₀ (mg l⁻¹)

Sediment dwelling organisms - Acute 96 hour LC₅₀ (mg l⁻¹)

Sediment dwelling organisms - Chronic 28 day NOEC, static, water (mg l⁻¹)

Sediment dwelling organisms - Chronic 28 day NOEC, sediment (mg kg⁻¹)

Aquatic plants - Acute 7 day EC₅₀, biomass (mg l⁻¹)

Algae - Acute 72 hour EC₅₀, growth (mg l⁻¹)

Algae - Chronic 96 hour NOEC, growth (mg l⁻¹)

Mesocosm study data

NOEAEC mg l⁻¹

HUMAN HEALTH AND PROTECTION

General

Property

Value

Source; quality score; and other information

Interpretation

Threshold of Toxicological Concern (Cramer Class)

High (class III)

Mammals - Acute oral LD₅₀ (mg kg⁻¹)

> 1800

Mouse as the digluconate

Moderate

Mammals - Dermal LD₅₀ (mg kg⁻¹ body weight)

500

Rat

Mammals - Inhalation LC₅₀ (mg l⁻¹)

> 2.03

Rat

Other Mammal toxicity endpoints

Subcutaneous LD₅₀ = 1000 mg kg⁻¹

Rat

Intraperitoneal LD₅₀ = 60 mg kg⁻¹

Rat

ADI - Acceptable Daily Intake (mg kg⁻¹ bw day⁻¹)

ARfD - Acute Reference Dose (mg kg⁻¹ bw day⁻¹)

AAOEL - Acute Acceptable Operator Exposure Level (mg kg⁻¹ bw day⁻¹)

AOEL - Acceptable Operator Exposure Level - Systemic (mg kg⁻¹ bw day⁻¹)

Dermal penetration studies (%)

Dangerous Substances Directive 76/464

Exposure Routes

Public

Occupational

Mammalian dose elimination route and rate

Excretion is primarily through the faeces (~ 90%). Also found in breast milk.

Health issues

Specific human health issues

Carcinogen

Genotoxic

Endocrine disruptor

No data found

; ; ; ;

No data found

Reproduction / development effects

Acetyl cholinesterase inhibitor

Neurotoxicant

No data found

Respiratory tract irritant

Skin irritant

Skin sensitiser

Eye irritant

Phototoxicant

No data found

General human health issues

No further information available

Handling issues

Property

Value and interpretation

General

May decompose on heating emiting dangerous gaseous substances - halogenated compounds, oxides of nitrogen
IMDG Transport Hazard Class 9

CLP classification 2013

WHO Classification

Not listed (Not listed)

UN Number

UN3077

Waste disposal & packaging

Packaging Group III (minor danger)

Shelf-life, storage, stability and reactivity

TRANSLATIONS

Language

Name

English

chlorhexidine

French

German

Danish

Italian

Spanish

clorhexidina

Greek

Polish

Swedish

Hungarian

Dutch

Norwegian

Record last updated:	30/08/2023
Contact:	aeru@herts.ac.uk
Please cite as:	Lewis, K.A., Tzilivakis, J., Warner, D. and Green, A. (2016) An international database for pesticide risk assessments and management. Human and Ecological Risk Assessment: An International Journal, 22(4), 1050-1064. DOI: 10.1080/10807039.2015.1133242