Bacillus firmus I-1582

Bacillus firmus I-1582	Last updated: 25/10/2025
(Not known by any other names)

Hazard alerts

The following alerts are based on the data in the tables below. An absence of an alert does not imply the substance has no implications for human health, biodiversity or the environment but just that we do not have the data to form a judgement. These hazard alerts do not take account of usage patterns or exposure, thus do not represent risk.

Environmental fate	Ecotoxicity	Human health
		Human health Low alert

GENERAL INFORMATION

Description

A gram-positive bacterium isolated from cultivated soil used to control damaging plant parasitic nematodes

Example pests controlled

Plant-parasitic nematodes including the cyst nematode Heterodera schachtii and root-knot nematodes (Meloidogyne spp.)

Example applications

Vegetables; Fruit; Herbs; Cotton; Ornamentals; Turf; Glasshouse crops

Efficacy & activity

Appearance and life cycle

An aerobic, Gram-positive, rod-shaped species

Taxonomic classification

Class: Bacilli; Order: Bacillales; Family: Bacillaceae

GB regulatory status

GB COPR regulatory status

Approved

Date COPR inclusion expires

30/09/2026

GB LERAP status

None

EC Regulation 1107/2009 (repealing 91/414)

EC Regulation 1107/2009 status

Not approved

Dossier rapporteur/co-rapporteur

France

Date EC 1107/2009 inclusion expires

EU Candidate for substitution (CfS)

Listed in EU database

Yes

Approved for use (✓) under EC 1107/2009 in the following EU Member States

ATAustria	BEBelgium	BGBulgaria	CYCyprus	CZCzech Republic	DEGermany	DKDenmark	EEEstonia	ELGreece

ESSpain	FIFinland	FRFrance	HRCroatia	HUHungary	IEIreland	ITItaly	LTLithuania	LULuxembourg

LVLatvia	MTMalta	NLNetherlands	PLPoland	PTPortugal	RORomania	SESweden	SISlovenia	SKSlovakia

Approved for use (✓) under EC 1107/2009 by Mutual Recognition of Authorisation and/or national regulations in the following EEA countries

ISIceland	NONorway

Additional information

Known to be also used in the following countries

Chemical structure

Isomerism

Not applicable

Chemical formula

Canonical SMILES

Isomeric SMILES

International Chemical Identifier key (InChIKey)

International Chemical Identifier (InChI)

2D structure diagram/image available?

General status

Biopesticide type

Nematicide

Substance groups

Micro-organism

Minimum active substance purity

Known relevant impurities

Substance origin

Natural

Mode of action

Actual mode of action is complex using various mechanisms including enzymatic action, degradation of root exudates and the production of a phytohormone.

Substance source

A naturally occurring soil bacterium which possesses nematicidal activity. Strain originally isolated from agricultural soil in Israel

Uses

Crop protection; Leisure - golf courses

Target pests

Plant-parasitic nematodes including the cyst nematode Heterodera schachtii and root-knot nematodes (Meloidogyne spp.)

Target host

Vegetables; Fruit; Herbs; Cotton; Ornamentals; Turf

Farming system suitability

Suitable for use inall farming systems where approved for use in that country

CAS RN

EC number

CIPAC number

US EPA chemical code

029072

PubChem CID

Molecular mass

Chemical name

Bacillus firmus isolate 1582

PIN (Preferred Identification Name)

IUPAC name

CAS name

Listed (✓) under

UK Poisons List Order 1972	Rotterdam Convention	Montreal Protocol
XNo The Poisons List Order 1972	XNo Rotterdam Convention	XNo Montreal Protocol
Stockholm Convention	OSPAR	EU Water Framework Directive
XNo Stockholm Convention	XNo OSPAR	XNo EU Water Framework Directive

Relevant Environmental Water Quality Standards

Forever chemical

Highly Hazardous Pesticide (HHP)

Type I

Type II

Other status information

Herbicide Resistance Class (HRAC MoA class)

Not applicable

Herbicide Resistance Class (WSSA MoA class)

Not applicable

Insecticide Resistance Class (IRAC MoA class)

Not applicable

Fungicide Resistance Class (FRAC MOA class)

Not applicable

Examples of recorded resistance

Physical state

Bacterium

Related substances & organisms

clothianidin

Commercial

Property

Value

Availability status

Current

Introduction & key dates

Circa 2000, introduced

Example manufacturers & suppliers of products using this active now or historically

Agrogreen Biological Division
Bayer CropScience

Example products using this active

Chancellor
Poncho Votivo
Nortica

Formulation and application details

Available in a variety of formulations including dry powders and those for use as drenches, sprays and with drip irrigation systems

Commercial production

Commercial production of Bacillus species typically involves large-scale aerobic fermentation. The process begins with selecting a robust strain, often genetically optimised for yield and stability. These bacteria are cultivated in bioreactors under tightly controlled conditions, temperature (usually 30–37 degrees C), pH (neutral to slightly alkaline), and oxygen levels are carefully regulated to maximise growth and productivity. Nutrient-rich media, often containing glucose or other carbon sources, support rapid biomass accumulation. After fermentation, the culture is harvested, and the desired products, such as enzymes or spores, are extracted and purified.

Impact on climate of production and use

As microbial-based products tend to use fermentation-based production processes rather than chemical synthesis, they typically have a lower fossil fuel input in formulation and active ingredient creation, and also have reduced downstream emissions due to biodegradability and minimal soil disruption, their life-cycle GHG emissions are expected to be low. Whilst hard and precise data is not available, broad estimates suggest that typically emissions are likely to be below 5 kg CO₂e/kg. As an example, an ISO 14044-compliant cradle-to-gate LCA undertaken in 2023 for a Bacillus firmus-based microbial seed treatment nematicide determined GHG emissions at 1.9 kg CO₂e/kg.

ENVIRONMENTAL FATE

Property

Value

Source; quality score; and other information

Interpretation

Solubility - In water at 20 °C at pH 7 (mg l⁻¹)

Solubility - In organic solvents at 20 °C (mg l⁻¹)

Melting point (°C)

Boiling point (°C)

Degradation point (°C)

Flashpoint (°C)

Octanol-water partition coefficient at pH 7, 20 °C

Log P

Fat solubility of residues

Solubility

Data type

Density (g ml⁻¹)

Dissociation constant pKa) at 25 °C

Vapour pressure at 20 °C (mPa)

Henry's law constant at 25 °C (Pa m³ mol⁻¹)

Volatilisation as max % of applied dose lost

From plant surface

From soil surface

Maximum UV-vis absorption L mol⁻¹ cm⁻¹

Surface tension (mN m⁻¹)

Degradation

Property

Value

Source; quality score; and other information

Interpretation

General biodegradability

Soil degradation (days) (aerobic)

DT₅₀ (typical)

DT₅₀ (lab at 20 °C)

DT₅₀ (field)

DT₉₀ (lab at 20 °C)

DT₉₀ (field)

DT₅₀ modelling endpoint

Note

Soil persistence is dependant on the availability of nutrients within the rhizosphere

Dissipation rate RL₅₀ (days) on plant matrix

Value

Note

Dissipation rate RL₅₀ (days) on and in plant matrix

Value

Note

Aqueous photolysis DT₅₀ (days) at pH 7

Value

Note

Aqueous hydrolysis DT₅₀ (days) at 20 °C and pH 7

Value

Note

Water-sediment DT₅₀ (days)

Water phase only DT₅₀ (days)

Sediment phase only DT₅₀ (days)

Air degradation

As this parameter is not normally measured directly, a surrogate measure is used: ‘Photochemical oxidative DT₅₀’. Where data is available, this can be found in the Fate Indices section below.

Decay in stored produce DT₅₀

Soil adsorption and mobility

Property

Value

Source; quality score; and other information

Interpretation

Linear

K_d (mL g⁻¹)

K_oc (mL g⁻¹)

Notes and range

Soil mobility depends on soil conditions and water availability

Freundlich

K_f (mL g⁻¹)

K_foc (mL g⁻¹)

¹/_n

Notes and range

pH sensitivity

Known metabolites

None

ECOTOXICOLOGY

Terrestrial ecotoxicology

Property

Value

Source; quality score; and other information

Interpretation

Mammals - Acute oral LD₅₀ (mg kg⁻¹)

No adverse effects identified or expected

Mammals - Short term dietary NOEL

(mg kg⁻¹)

No adverse effects identified or expected

(ppm diet)

Mammals - Chronic 21d NOAEL (mg kg⁻¹ bw d⁻¹)

Birds - Acute LD₅₀ (mg kg⁻¹)

No adverse effects identified or expected

Birds - Short term dietary (LC₅₀/LD₅₀)

No adverse effects identified or expected

Birds - Chronic 21d NOEL (mg kg⁻¹ bw d⁻¹)

Earthworms - Acute 14 day LC₅₀ (mg kg⁻¹ dw soil)

> 3.51 x 10⁰⁹

Eisenia foetida as spores/Kg

Earthworms - Chronic NOEC, reproduction (mg kg⁻¹ dw soil)

Soil micro-organisms

Collembola

Acute LC₅₀ (mg kg⁻¹)

Chronic NOEC (mg kg⁻¹)

Non-target plants

Vegetative vigour ER₅₀ (g ha⁻¹)

Seedling emergence ER₅₀ (g ha⁻¹)

Honeybees (Apis spp.)

Contact acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Oral acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

> 3.51 x 10⁰⁵

as spores/bee

Unknown mode acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Chronic

Notes

Bumblebees (Bombus spp.)

Contact acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Oral acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Mason bees (Osmia spp.)

Contact acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Oral acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg bee⁻¹)

Other bee species (1)

Acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg insect⁻¹)

Mode of exposure

Other bee species (2)

Acute LD₅₀ (worst case from 24, 48 and 72 hour values - μg insect⁻¹)

Mode of exposure

Beneficial insects (Ladybirds)

Beneficial insects (Lacewings)

Beneficial insects (Parasitic wasps) as NOEC spores/Kg

2.8 x 10¹⁴

Aphidius rhopalosiphi

Beneficial insects (Predatory mites)

Beneficial insects (Ground beetles)

Aquatic ecotoxicology

Property

Value

Source; quality score; and other information

Interpretation

Temperate Freshwater Fish - Acute 96 hour LC₅₀ (mg l⁻¹)

> 1.05 x 10¹⁰

Oncorhynchus mykiss as CFU/L

Temperate Freshwater Fish - Chronic 21 day NOEC (mg l⁻¹)

No adverse effects identified or expected

Tropical Freshwater Fish - Acute 96 hour LC₅₀ (mg l⁻¹)

Temperate Freshwater Aquatic invertebrates - Acute 48 hour EC₅₀ (mg l⁻¹)

> 1.5 x 10¹⁰

Daphnia magna as CFU/L

Temperate Freshwater Aquatic invertebrates - Chronic 21 day NOEC (mg l⁻¹)

1.38 x 10⁰⁵

Daphnia magna as CFU/L

Tropical Freshwater Aquatic invertebrates - Acute 48 hour EC₅₀ (mg l⁻¹)

Aquatic crustaceans - Acute 96 hour LC₅₀ (mg l⁻¹)

Sediment dwelling organisms - Acute 96 hour LC₅₀ (mg l⁻¹)

Sediment dwelling organisms - Chronic 28 day NOEC, static, water (mg l⁻¹)

Sediment dwelling organisms - Chronic 28 day NOEC, sediment (mg kg⁻¹)

Aquatic Plants (free-floating, fonds growth, fresh) - 7 day (mg l⁻¹)

No adverse effects identified or expected

Aquatic plants (rooted, growth rate, fresh) - 14 day (mg l⁻¹)

Algae - Acute (growth rate, fresh; mg l⁻¹) (EbC50 (biomass))

20.6 x 10⁰⁸

Unknown species as CFU/L

Algae - Chronic (growth rate, fresh; mg l⁻¹)

No adverse effects identified or expected

Mesocosm study data

NOEAEC mg l⁻¹

Marine bivalves

HUMAN HEALTH AND PROTECTION

General

Property

Value

Source; quality score; and other information

Interpretation

Threshold of Toxicological Concern (Cramer Class)

Not applicable

Mammals - Acute oral LD₅₀ (mg kg⁻¹)

No adverse effects identified or expected

Mammals - Dermal LD₅₀ (mg kg⁻¹ body weight)

> 5050

Rat

Mammals - Inhalation LC₅₀ (mg l⁻¹)

Other Mammal toxicity endpoints

ADI - Acceptable Daily Intake (mg kg⁻¹ bw day⁻¹)

ARfD - Acute Reference Dose (mg kg⁻¹ bw day⁻¹)

AAOEL - Acute Acceptable Operator Exposure Level (mg kg⁻¹ bw day⁻¹)

AOEL - Acceptable Operator Exposure Level - Systemic (mg kg⁻¹ bw day⁻¹)

Dermal penetration studies (%)

Dangerous Substances Directive 76/464

Exposure Routes

Public

Occupational

Mammalian dose elimination route and rate

Health issues

Specific human health issues (hazard-based)

Carcinogen

Genotoxic

Endocrine disruptor

; ; ; ;

Reproduction / development effects

Acetyl cholinesterase inhibitor

Neurotoxicant

Respiratory tract irritant

Skin irritant

Skin sensitiser

Eye irritant

Phototoxicant

No data found

General human health issues

Not expected to be harmful to humans
Micro-organisms may cause sensitising reactions

Handling issues

Property

Value and interpretation

General

No information available

CLP classification 2013

Not classified as hazardous

WHO Classification

Not listed (Not listed)

UN Number

Waste disposal & packaging

Shelf-life, storage, stability and reactivity

TRANSLATIONS

Language

Name

English

Bacillus firmus I-1582

French

Bacillus firmus

German

Bacillus firmus

Danish

Bacillus firmus

Italian

Bacillus firmus

Spanish

Bacillus firmus

Greek

Bacillus firmus

Polish

Bacillus firmus

Swedish

Bacillus firmus

Hungarian

Bacillus firmus

Dutch

Bacillus firmus

Norwegian

Record last updated:	25/10/2025
Contact:	aeru@herts.ac.uk
Please cite as:	Lewis, K.A., Tzilivakis, J., Warner, D. and Green, A. (2016) An international database for pesticide risk assessments and management. Human and Ecological Risk Assessment: An International Journal, 22(4), 1050-1064. DOI: 10.1080/10807039.2015.1133242